奥尔加Pakhomova
RESEARCH Associate Professor
电话: +1-757-683-8006
电子邮件: opakhomo@corradopremuda.com
地址: IRP 2, 4211 Monarch Way, Norfolk, VA, 23508
实验室网站Education
- Postdoctoral RESEARCH fellow: University of Texas 健康 Science Center, Texas, USA, 1999-2009
- 博士: in Radiation Biology/Biophysics, Medical Radiology RESEARCH Center 俄罗斯n Academy of Medical Sciences (MRRC-RAMS), 俄罗斯. 1995
- M.S: in Microbiology, Moscow State University, Moscow, USSR. 1982
实验室成员
Giedre Silkuniene, 做博士后RESEARCH
RESEARCH描述
We have studied mechanisms governing cell death and recovery after electric injury for more than ten years. The recent research focuses on the membrane protein machinery recognizing electric stimulation, 促进损伤, and participating in plasma membrane repair.
Electric field (EF) causes defects in the cell plasma membrane, the process called electroporation. The electroporation mechanism involves a complex interaction between membrane lipids and membrane proteins. Mechanisms that mediate the impacts of proteins are weakly understood. Our group identifies and characterizes membrane proteins that are pivotal for cell response to EF application. We proved that the TMEM16F ion channel is an essential modulator of cell physiology downstream of EF injury. The extensive search for other vital modulators is in progress. 也, we explore membrane healing machinery, which points to therapy pursuing membrane recovery after electric injury.
各种生化, molecular biology approaches, fluorescent life microscopy, single-cell electrophysiology are available in the laboratory and implemented in the research.
Ongoing research projects:
- Mechanisms of cell membrane repair after electropermeabilization
- Mechanisms of voltage-gated calcium channel activation by nsPEF
- Identification of nsPEF-sensitive endogenous ion channels
- Engagement of ion channels into the downstream cytophysiological effects of nsPEF.
网站登录
Most relevant publications
- Bhattacharya S, Silkunas М, Gudvangen Е, Mangalanathan U, Pakhomova上， pakhomv AG. Ca2+ dependence and kinetics of cell membrane repair after electropermeabilization, Biochimica et Biophysica Acta (BBA) - Biomembranes, 2022, 卷1864, 问题2, 183823
- Pakhomov AG) Pakhomova上. The interplay of excitation and electroporation in nanosecond pulse stimulation [published online ahead of print, 2020年7月15日]. 生物电化学. 2020: 136, 107598.
- Michel O， pakhomv AG, Casciola M, Saczko J, Kulbacka J, Pakhomova上. Electropermeabilization does not correlate with plasma membrane lipid oxidation. 生物电化学. 2020: 132, 107433.
- Hristov K, Mangalanathan U, Casciola M, Pakhomova上， pakhomv AG. Expression of voltage-gated calcium channels augments cell susceptibility to membrane disruption by nanosecond pulsed electric field. Biochim Biophys Acta Biomembr. 2018 Nov; 1860(11):2175-2183.
- Muratori C， pakhomv AG, Gianulis E, Meads J, Casciola M, Mollica PA, Pakhomova上. Activation of the phospholipid scramblase TMEM16F by nanosecond pulsed electric fields (nsPEF) facilitates its diverse cytophysiological effects. 生物化学. 2017 Nov 24;292(47):19381-19391.
奖项及荣誉
- 2019 Invited lecturer, Radiation Biology School, Obninsk, 俄罗斯
- 2020 Tenure in Fulbright Specialist Program (2/2020-2/2024)
- 2020 Invited lecturer, 4th 国际 Wroclaw Scientific School, October 9-10, 2020. 波兰弗罗茨瓦夫